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Hsin-Yang Chang Associate Professor

張欣暘 副教授
Hsin-Yang Chang,

Assistant Professor,2015.8

Ph.D. (2010),University of Illinois at Urbana-Champaign


Email: hychang@mail.nsysu.edu.tw





Enzyme structural biology; Industrial enzymes studies;
Algae/plant membrane protein biogenesis; Protein chemistry;

1. Chang HY*, Lin ST, Ko TP, Wu SM, Lin TH, Chang YC, Huang KF and Lee TM* (2017) Enzymatic characterization and crystal structure analysis of Chlamydomonas reinhardtii dehydroascorbate reductase and their implications for oxidative stress, Plant Physiol. Biochem. 120, 144-155. (SCI)
2. Manuel Maestre-Reyna, Shu-Mei Wu, Yu-Ching Chang, Chi-Chih Chen, Alvaro Maestre-Reyna, Andrew H.-J. Wang, Chang HY* (2017) In search of tail-anchored protein machinery in plants: reevaluating the role of arsenite transporters, Scientific Report 7:46022 | DOI: 10.1038/srep46022 (SCI)
3. Chang HY, Chou CC, WuML, Wang AHJ. (2017) Expression, Purification and Enzymatic Characterization of Undecaprenyl Pyrophosphate Phosphatase from Vibrio vulnificus, Protein Expr. Purif. 133:121-131 (SCI)
4. Ballmoos CV, Smirnova I, Poiana F, Gonska N, Chang HY, Gennis RB, Brzezinski P, and Pia A¨ delroth (2017) Dynamics of the KB Proton Pathway in Cytochrome ba3 from Thermus thermophiles, Isr. J. Chem. DOI: 10.1002/ijch.201600136 (SCI)
5. Lin ST, Chiou CW, Chu YL, Hsiao Y, Tseng YF, Chen YC, Chen HJ, Chang HY, Lee TM. (2016) Enhanced Ascorbate Regeneration via Dehydroascorbate Reductase Confers Tolerance to Photo-Oxidative Stress in Chlamydomonas reinhardtii. Plant Cell Physiol. 57(10):2104-2121 (SCI)
6. Mateja A, Paduch M, Chang Hsin-Yang, Szydlowska A, Kossiakoff A, Hegde R, Keenan R. (2015) Structure of the Get3 targeting factor in complex with its membrane protein cargo. Science. 347: 1152-1155. (IF: 30.5; Ranking: 2/55 in MULTIDISCIPLINARY SCIENCES)
7. Chang Hsin-Yang, Chou CC, Hsu MF, Wang AH. (2014) Proposed carrier lipid-binding site of undecaprenyl pyrophosphate phosphatase from Escherichia coli. J. Biol. Chem. 289: 18719-18735. (IF: 5.0; Ranking: 66/289 in BIOCHEMISTRY & MOLECULAR BIOLOGY)
8. Smirnova I, Chang Hsin-Yang, von Ballmoos C, Ädelroth P, Gennis RB, Brzezinski P. (2013) Single mutations that redirect internal proton transfer in the ba3 oxidase from Thermus thermophilus. Biochemistry 52: 7022-7030. (IF: 3.5; Ranking: 106/289 in BIOCHEMISTRY & MOLECULAR BIOLOGY)
9. Chang Hsin-Yang, Choi SK, Vakkasoglu AS, Chen Y, Hemp J, Fee JA, Gennis RB. (2012) Exploring the proton pump and exit pathway for pumped protons in cytochrome ba3 from Thermus thermophilus. Proc Natl Acad Sci U S A 109: 5259-5264. (IF: 10.5; Ranking: 3/55 in MULTIDISCIPLINARY SCIENCES)
10. Chang Hsin-Yang, Ann Y, Pace L, Lin MT, Lin YH, Gennis RB. (2010) The diheme cytochrome c4 from Vibrio cholerae is a natural electron donor to the cbb3-type cytochrome c oxidase. Biochemistry 49: 7494-7503. (IF: 3.5; Ranking: 106/289 in BIOCHEMISTRY & MOLECULAR BIOLOGY)
11. Chang Hsin-Yang, Hemp J, Chen Y, Fee JA, Gennis RB. (2009) The cytochrome ba3 oxygen reductase from Thermus thermophilus uses a single input channel for proton delivery to the active site and for proton pumping. Proc Natl Acad Sci U S A 106: 16169-16173. (IF: 10.5; Ranking: 3/55 in MULTIDISCIPLINARY SCIENCES)
12. Smirnova I, Reimann J, von Ballmoos C, Chang Hsin-Yang, Gennis RB, Fee JA, Brzezinski P, Adelroth P. (2010) Functional role of Thr-312 and Thr-315 in the proton-transfer pathway in ba3 cytochrome c oxidase from Thermus thermophilus. Biochemistry 49: 7033-7049. (IF: 3.5; Ranking: 106/289 in BIOCHEMISTRY & MOLECULAR BIOLOGY)
13. Kuo TH, Chuang SC, Chang Sing-Yang, Liang PH.(2006) Ligand specificities and structural requirements of two Tachypleus plasma lectins for bacterial trapping. Biochem. J. 393: 757-766. (IF: 4.8; Ranking: 61/289 in BIOCHEMISTRY & MOLECULAR BIOLOGY)
14. Chen AP, Chang Sing-Yang, Lin YC, Sun YS, Chen CT, Wang AH, Liang PH. (2005) Substrate and product specificities for cis-type undecaprenyl pyrophosphate synthase. Biochem. J. 386: 169-176. (IF: 4.8; Ranking: 61/289 in BIOCHEMISTRY & MOLECULAR BIOLOGY)
15. Chang Sing-Yang, Ko TP, Chen AP, Wang AH, Liang PH. (2004) Substrate binding mode and reaction mechanism of undecaprenyl pyrophosphate synthase deduced from crystallographic studies. Protein Sci. 13: 971-978. (IF: 3.0; Ranking: 153/289 in BIOCHEMISTRY & MOLECULAR BIOLOGY)
16. Chang Sing-Yang, Ko TP, Liang PH, Wang AH. (2003) Catalytic mechanism revealed by the crystal structure of undecaprenyl pyrophosphate synthase in complex with sulfate, magnesium, and Triton. J. Biol. Chem. 278: 29298-29307. (IF: 5.0; Ranking: 66/289 in BIOCHEMISTRY & MOLECULAR BIOLOGY)
17. Chang Sing-Yang, Chen YK, Wang AH, Liang PH. (2003) Identification of the active conformation and the importance of length of the flexible loop 72-83 in regulating the conformational change of undecaprenyl pyrophosphate synthase. Biochemistry. 42: 14452-14459. (IF: 3.5; Ranking: 106/289 in BIOCHEMISTRY & MOLECULAR BIOLOGY)
18. Atreya CE, Johnson EF, Williamson J, Chang Sing-Yang, Liang PH, Anderson KS. (2003). Probing electrostatic channeling in protozoal bifunctional thymidylate synthase-dihydrofolate reductase using site-directed mutagenesis. J. Biol. Chem. 278: 28901-28911. (IF: 5.0; Ranking: 66/289 in BIOCHEMISTRY & MOLECULAR BIOLOGY)
19. Chang Sing-Yang, Tsai PC, Tseng CS, Liang PH. (2001) Refolding and characterization of a yeast dehydrodolichyl diphosphate synthase overexpressed in Escherichia coli. Prot. Exp. Puri. 23: 432-439. (IF: 1.6; Ranking: 227/289 in BIOCHEMISTRY & MOLECULAR BIOLOGY)




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